Perbandingan Metode Ekstraksi DNA Guanidium isothiocyanate dan Filtrasi tube terhadap Kualitas Hasil Pemeriksaan PCR Plasmodium falciparum

Edy Kurniawan; Pipin Amalia; Hardin Hardin

doi:10.62588/otusedu.2025.v3i3.0290

Authors

Edy Kurniawan 1

Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains dan Teknologi Universitas Bima Internasional MFH
Pipin Amalia 2

Program Studi Diploma Tiga Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Sains dan Teknologi Universitas Bima Internasional MFH
Hardin Hardin 3

Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains dan Teknologi Universitas Bima Internasional MFH

Abstract

This study aims to compare the results of Plasmodium falciparum detection via PCR using two DNA extraction methods: the Guanidium isothiocyanate method (with phenol-chloroform) and the tube filtration method (Spin column). The research method used was a descriptive survey with a retrospective research design. This study was retrospective, using two samples of positive malaria patients from the NTB Provincial General Hospital. PCR results were analyzed using agarose gel electrophoresis to identify DNA bands. The results showed that the tube filtration method produced brighter and clearer DNA bands, while the Guanidium isothiocyanate method produced less clear bands. This indicates that the tube filtration method is more effective in producing pure DNA that can be detected well by PCR. It can be concluded that the tube filtration method is superior because it is practical, safe, and the accuracy of the results of Plasmodium falciparum DNA detection is of higher quality than the Guanidium isothiocyanate method.

Keywords:

DNA Extraction, Method, PCR, Plasmodium falciparum

DOI:

https://doi.org/10.62588/otusedu.2025.v3i3.0290

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